Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR
نویسندگان
چکیده
منابع مشابه
identification of legionella pneumophila in intubated patients with taqman real time pcr
background legionellaceae contains legionella genus with over 52 species and 64 serogroups. it is one of the most important causes of respiratory disease in human. more than 30% of hospital-acquired pneumonia is caused by legionella. ventilator-associated pneumonia (vap) is an infection acquired in hospital wards, particularly in intensive care unit (icu). this disease approximately affects 9% ...
متن کاملIdentification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR
BACKGROUND Legionellaceae contains Legionella genus with over 52 species and 64 serogroups. It is one of the most important causes of respiratory disease in human. More than 30% of hospital-acquired pneumonia is caused by Legionella. Ventilator-associated pneumonia (VAP) is an infection acquired in hospital wards, particularly in intensive care unit (ICU). This disease approximately affects 9% ...
متن کاملIdentification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.
Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.
متن کاملClinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1.
We developed a single-tube multiplex real-time PCR assay capable of simultaneously detecting and discriminating Legionella spp., Legionella pneumophila, and Legionella pneumophila serogroup 1 in primary specimens. Evaluation of 21 clinical specimens and 115 clinical isolates demonstrated this assay to be a rapid, high-throughput diagnostic test with 100% specificity that may aid during legionel...
متن کاملDevelopment of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2015
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.15094